pEXT09/h
Cat# exv54 pPICZ-His/C Vector Infomation
  
  5X EX-Buffer
EX-Vector
EXclonase Enzyme
Ship and Storage
40 µl
20 µl
10 µl
-20 oC

Vector pEXT09/h (pPICZ-His/C) is modified from plasmid pPICZ  which carry an C-terminal His tag, and driven by AOX1 promoter.

   
Purpose:Protein expression in Yeast
Backbone:pPICZ
Promoter:AOX1
Size (bp):4070
C-ter Tag(s):His
Type:Protein expression
5' primer:5' AOX1 primer
3' primer:3' AOX1 primer
Resistance(s):Zeocin
Description
pPICZ-alpha vector was designed to express recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to a C-terminal peptide containing the c-myc epitope and a polyhistidine (6xHis) tag. The vector allows highlevel, methanol inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain including X-33, GS115, SMD1168H, and KM71H.
Reference
Pichia expression vectors for selection on Zeocin and purification of recombinant proteins
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