pEXT10/H
Cat# exv51 pFastBac-His/N Vector Infomation
  
  5X EX-Buffer
EX-Vector
EXclonase Enzyme
Ship and Storage
40 µl
20 µl
10 µl
-20 oC

Vector pEXT10/H (pFastBac-His/N) is modified from plasmid pFastBac  which carry an N-terminal His tag, and driven by PH promoter.

   
Purpose:Protein expression in insect cells
Backbone:pFastBac
Promoter:PH
Size (bp):5585
N-ter Tag(s):His
Type:Protein expression
5' primer:pFastbac-F
3' primer:pFastbac-R
Resistance(s):Ampicillin
Description
The expression of the gene of interest is controlled by the Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) polyhedrin (PH) promoter for high-level expression in insect cells. This expression cassette is flanked by the left and right arms of Tn7, and also contains a gentamicin resistance gene and an SV40 polyadenylation signal to form a mini Tn7.
Reference
Bac-to-Bac Baculovirus Expression Systems
杭州百格生物技术有限公司 www.biogle.cn